Quantitative PCR Service of IBR Inc.

Quantitative polymerase chain reaction (qPCR) is the method of choice for the quantitative determination of target DNA or RNA distribution and expression.
With the Applied Biosystems 7500 Real-Time PCR System IBR Inc. is capable to detect and quantitate the distribution and expression of human, animal, plant, bacterial and viral genes. The scientists of IBR Inc. support your projects from assay development to validation and sample measurement in compliance with IBR Inc. quality standards.

Quantitative PCR Applications

IBR Inc. is versed in the following qPCR applications:

  • Host residual DNA quantitation
  • Gene expression analysis
  • Drug response analysis of gene expression
  • Chemosensitivity profiling of cancer drugs
  • Mutation analysis

Our PCR-Service

Our qPCR-Service includes:

  • DNA and RNA extraction including quantitation by spectrometry (NanoDrop)
  • cDNA synthesis
  • Primer design
  • Design of probes and quencher probes
  • Quantitative PCR development and validation
  • Quantitative reverse transcriptase PCR (RT-PCR) development and validation 
  • Sample analysis

The 7500 Real Time PCR System of IBR Inc.

For qPCR we employ the Applied Biosystems 7500 Real-Time PCR System which simultaneously measures more than one fluorescence wavelength per well. Thus, endogenous controls can be run either as parallel reactions in separate wells or within the same well as the sample.

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